Universal PCR amplification of mouse immunoglobulin gene variable regions: the design of degenerate primers and an assessment of the effect of DNA polymerase 3′ to 5′ exonuclease activity

Author:

Wang Zhongde,Raifu Murisiku,Howard Meredith,Smith Laurie,Hansen David,Goldsby Richard,Ratner David

Publisher

Elsevier BV

Subject

Immunology,Immunology and Allergy

Reference24 articles.

1. Rescue of a failed polymerase chain reaction catalyzed by Vent or deep Vent DNA polymerase with 10% dimethyl sulfoxide;Berger;Anal. Biochem.,1994

2. Direct cDNA cloning of the rearranged immunoglobulin variable region;Chiang;Biotechniques,1989

3. PCR fidelity of pfu DNA polymerase and other thermostable DNA polymerases;Cline;Nucleic Acids Res.,1996

4. Primer design for the cloning of immunoglobulin heavy-chain leader-variable regions from mouse hybridoma cells using the PCR;Coloma;Biotechniques,1991

5. Rapid cloning of any rearranged mouse immunoglobulin variable genes;Dattamajumdar;Immunogenetics,1996

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