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Multicenter quality assessment of 16S ribosomal DNA-sequencing for microbiome analyses reveals high inter-center variability

  • Published:2016-08 Issue:5 Volume:306 Page:334-342
  • ISSN:1438-4221
  • Container-title:International Journal of Medical Microbiology
  • language:en
  • Short-container-title:International Journal of Medical Microbiology

Author:

Hiergeist Andreas,Reischl Udo,Gessner Andrè

Funder

INSTAND

German Research Foundation

Publisher

Elsevier BV

Subject

Infectious Diseases,Microbiology (medical),General Medicine,Microbiology

Reference37 articles.

1. Aronesty, E., Ea-utils: command-line tools for processing biological sequencing data. 2011 https://code.google.com/archive/p/ea-utils/.

2. Freezing fecal samples prior to DNA extraction affects the Firmicutes to Bacteroidetes ratio determined by downstream quantitative PCR analysis;Bahl;FEMS Microbiol. Lett.,2012

3. QIIME allows analysis of high-throughput community sequencing data;Caporaso;Nat. Methods,2010

4. A comparison of methods for clustering 16S rRNA sequences into OTUs;Chen;PLoS One,2013

5. Ribosomal database project: data and tools for high throughput rRNA analysis;Cole;Nucleic Acids Res.,2014
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