Enterokinase monolithic bioreactor as an efficient tool for biopharmaceuticals preparation: on-line cleavage of fusion proteins and analytical characterization of released products

Author:

Tengattini Sara,Rinaldi Francesca,Piubelli LucianoORCID,Kupfer Tom,Peters Benjamin,Bavaro Teodora,Calleri Enrica,Massolini Gabriella,Temporini Caterina

Funder

Fondazione Banca del Monte di Lombardia

Publisher

Elsevier BV

Subject

Clinical Biochemistry,Spectroscopy,Drug Discovery,Pharmaceutical Science,Analytical Chemistry

Reference30 articles.

1. Thioredoxin and related proteins as multifunctional fusion tags for soluble expression in E. Coli;LaVallie;Methods Mol. Biol.,2003

2. Fusion Protein Technologies for Biopharmaceuticals: Applications and Challenges;Schmidt,2013

3. Recombinant protein expression and purification: a comprehensive review of affinity tags and microbial applications;Young;Biotechnol. J.,2012

4. Current strategies for the use of affinity tags and tag removal for the purification of recombinant proteins;Arnau;Protein Expr. Purif.,2006

5. An overview of enzymatic reagents for the removal of affinity tags;Waugh;Protein Expr. Purif.,2011

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