Recreation of in-host acquired single nucleotide polymorphisms by CRISPR-Cas9 reveals an uncharacterised gene playing a role in Aspergillus fumigatus azole resistance via a non-cyp51A mediated resistance mechanism-Reference-Cited by-同舟云学术

Recreation of in-host acquired single nucleotide polymorphisms by CRISPR-Cas9 reveals an uncharacterised gene playing a role in Aspergillus fumigatus azole resistance via a non-cyp51A mediated resistance mechanism

Published:2019-09 Issue: Volume:130 Page:98-106
ISSN:1087-1845
Container-title:Fungal Genetics and Biology
language:en
Short-container-title:Fungal Genetics and Biology

Author:

Ballard Eloise,Weber Jakob,Melchers Willem J.G.,Tammireddy Seshu,Whitfield Phillip D.,Brakhage Axel A.,Brown Alistair J.P.,Verweij Paul E.,Warris Adilia

Funder

Wellcome Trust Strategic Award

MRC Centre for Medical Mycology

Biotechnology and Biological Sciences Research Council

Publisher

Elsevier BV

Subject

Genetics,Microbiology

Reference54 articles.

1. Whole-genome sequencing reveals highly specific gene targeting by in vitro assembled Cas9-ribonucleoprotein complexes in Aspergillus fumigatus;Al Abdallah;Fungal Biol. Biotechnol.,2018

2. A simple and universal system for gene manipulation in Aspergillus fumigatus: in vitro-assembled Cas9-guide RNA ribonucleoproteins coupled with microhomology repair templates;Al Abdallah;mSphere,2017

3. Interrogation of related clinical pan-azole-resistant Aspergillus fumigatus strains: G138C, Y431C, and G434C single nucleotide polymorphisms in cyp51A, upregulation of cyp51A, and integration and activation of transposon Atf1 in the cyp51A promoter;Albarrag;Antimicrob. Agents Chemother.,2011