Nucleic acid amplification-free biosensor for sensitive and specific cfDNA detection based on CRISPR-Cas12a and single nanoparticle dark-field microscopy (DFM) imaging

Author:

Li Xianghui,Yang Xiulin,Pan Zhipeng,Zhuo Shuangmu,Lin Zhenyu,Chen Jianxin

Funder

National Natural Science Foundation of China

Natural Science Foundation of Fujian Province

Publisher

Elsevier BV

Subject

Materials Chemistry,Electrical and Electronic Engineering,Metals and Alloys,Surfaces, Coatings and Films,Condensed Matter Physics,Instrumentation,Electronic, Optical and Magnetic Materials

Reference20 articles.

1. Cell-free DNA (cfDNA): clinical significance and utility in cancer shaped by emerging technologies;Volik;Mol. Cancer Res.,2016

2. Toward the early detection of cancer by decoding the epigenetic and environmental fingerprints of cell-free DNA;van der Pol;Cancer Cell,2019

3. The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments;Bustin;Clin. Chem.,2009

4. Separation, purification, and detection of cfDNA in a microfluidic device;Kye;BioChip J.,2020

5. Tetrahedral DNA framework-programmed electrochemical biosenors with gold nanoparticles for ultrasensitive cell-free DNA detection;Wang;Nanomater,2022

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