Rapid purification of the over-expressed membrane 3 β-hydroxysteroid dehydrogenase in the presence of detergents
Author:
Publisher
Elsevier BV
Subject
Materials Chemistry,Inorganic Chemistry,Condensed Matter Physics
Reference35 articles.
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3. Structure, function and tissue-specific gene expression of 3β-hydroxysteroid dehydrogenase/5-ene-4-ene isomerase enzymes in classical and peripheral intracrine steroidogenic tissues
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1. Allosteric interaction between 3β‐hydroxysteroid dehydrogenase/Δ5‐Δ4isomerase and cytochrome b5influences cofactor binding;The FASEB Journal;2012-09-14
2. Crystallogenesis Research for Biology in the Last Two Decades as Seen from the International Conferences on the Crystallization of Biological Macromolecules;Crystal Growth & Design;2007-10-26
3. The engineered, cytosolic form of human type I 3beta-hydroxysteroid dehydrogenase/isomerase: purification, characterization and crystallization;Journal of Molecular Endocrinology;2001-08-01
4. Membrane-Bound Human 3β-Hydroxysteroid Dehydrogenase: Overexpression with His-Tag Using a Baculovirus System and Single-Step Purification;Protein Expression and Purification;2000-03
5. Creation of a fully active, cytosolic form of human type I 3beta-hydroxysteroid dehydrogenase/isomerase by the deletion of a membrane-spanning domain;Journal of Molecular Endocrinology;1999-10-01
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