Investigation of selective release of periplasmic proteins through pore size analysis and single-cell microscopy in Escherichia coli

Author:

Ramalakshmi Subbarayalu,Ramanan Ramakrishnan NagasundaraORCID,Madhavan Shanmugavel,Ooi Chien Wei,Chang Catherine Ching Han,Harper Ian S.ORCID,Lewis David M.,Lee Andrew K.,He Lizhong,Seenichamy Arivudainambi

Funder

Government of Malaysia Ministry of Higher Education

Government of Malaysia Ministry of Science Technology and Innovation

Publisher

Elsevier BV

Subject

Biomedical Engineering,Environmental Engineering,Bioengineering,Biotechnology

Reference43 articles.

1. Stepwise optimization of recombinant protein production in Escherichia coli utilizing computational and experimental approaches;Packiam;Appl. Microbiol. Biotechnol.,2020

2. Formation of giant unilamellar proteo-liposomes by osmotic shock;Motta;Langmuir.,2015

3. Extraction of recombinant periplasmic proteins under industrially relevant process conditions: selectivity and yield strongly depend on protein titer and methodology;Schimek;Biotechnol. Prog.,2020

4. The outer membrane is an essential load-bearing element in Gram-negative bacteria;Rojas;Nature.,2018

5. Integration of osmotic shock assisted liquid biphasic system for protein extraction from microalgae Chlorella vulgaris;Koyande;Biochem. Eng. J.,2020

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