Setup and characterization of a human head and neck squamous cell carcinoma xenograft model in nude rats-Reference-Cited by-同舟云学术

Setup and characterization of a human head and neck squamous cell carcinoma xenograft model in nude rats

Published:2006-12 Issue:6 Volume:135 Page:853-857
ISSN:0194-5998
Container-title:Otolaryngology–Head and Neck Surgery
language:en
Short-container-title:Otolaryngol Head Neck Surg

Author:

Bao Ande¹,Phillips William T.²,Goins Beth²,McGuff Howard S.³,Zheng Xiangpeng⁴,Woolley F. Ross²,Natarajan Mohan¹,Santoyo Cristina²,Miller Frank R.¹,Otto Randal A.¹

Affiliation:

1. Departments of Otolaryngology–Head and Neck Surgery, University of Texas Health Science Center at San Antonio, Texas

2. Department of Radiology, University of Texas Health Science Center at San Antonio, Texas

3. Department of Pathology, University of Texas Health Science Center at San Antonio, Texas

4. Department of Radiation Oncology, University of Texas Health Science Center at San Antonio, Texas

Abstract

OBJECTIVE: To develop and characterize a new head and neck cancer animal model. STUDY DESIGN: A human head and neck squamous cell carcinoma (HNSCC) xenograft model in nude rats was established via subcutaneous inoculation of a human-origin HNSCC cell line, SCC-4. The tumor was evaluated for growth characteristics, pathologic features by hematoxylin-eosin (HE) staining, and immunohistochemistry of epidermal growth factor receptor (EGFR). 2-[18F] fluoro-2-deoxy-D-glucose (18F-FDG) positron emission tomography (PET) imaging characteristics were studied too. RESULTS: A new HNSCC animal model was successfully established. Tumor sizes reached about 1 cm3 on day 15 after tumor cell inoculation. HE staining pathology has confirmed that this tumor is a typical SCC. EGFR immunohistochemistry demonstrated this tumor model to be strongly EGFR positive. 18F-FDG PET study has shown that 18F-FDG accumulated in tumors. CONCLUSIONS: This study has demonstrated that this tumor model is an appropriate HNSCC tumor model for animal studies on HNSCC.

Publisher

SAGE Publications

Subject

Otorhinolaryngology,Surgery

Link

http://journals.sagepub.com/doi/pdf/10.1016/j.otohns.2006.06.1257

Reference18 articles.

1. FDG–PET. A possible prognostic factor in head and neck cancer

2. Various immunostaining patterns of CD31, CD34 and endoglin and their relationship with lymph node metastasis in oral squamous cell carcinomas

3. Potential use of drug carried-liposomes for cancer therapy via direct intratumoral injection

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