Detection of human immunodeficiency virus type 1 in semen from seropositive men using culture and polymerase chain reaction deoxyribonucleic acid amplification techniques**Andrology/Urology Prize Paper. Presented at the 46th Annual Meeting of The American Fertility Society, Washington, D.C., October 13 to 18, 1990.††Supported by contract CSA-88-020 from the Contraceptive Research and Development (CONRAD) Program, under a cooperative agreement with the United States Agency of International Development (USAID), which in turn receives funds for AIDS research from an interagency agreement with the National Institute of Child Health and Human Development (NICHD), Bethesda, Maryland. The views expressed by the authors do not necessarily reflect the views of the USAID or CONRAD.