Contribution of cell culture, RNA extraction, and reverse transcription to the measurement error in quantitative reverse transcription polymerase chain reaction-based gene expression quantification-Reference-Cited by-同舟云学术

Contribution of cell culture, RNA extraction, and reverse transcription to the measurement error in quantitative reverse transcription polymerase chain reaction-based gene expression quantification

Published:2009-10 Issue:1 Volume:393 Page:29-35
ISSN:0003-2697
Container-title:Analytical Biochemistry
language:en
Short-container-title:Analytical Biochemistry

Author:

Combes Jean-Damien,Grelier Gaël,Laversanne Matthieu,Voirin Nicolas,Chabaud Sylvie,Ecochard René,Lasset Christine,Moyret-Lalle Caroline

Publisher

Elsevier BV

Subject

Cell Biology,Molecular Biology,Biochemistry,Biophysics

Reference54 articles.

1. Reliability of reverse transcription–polymerase chain reaction (RT–PCR)-based assays for the detection of circulating tumour cells: a quality-assurance initiative of the EORTC Melanoma Cooperative Group;Keilholz;Eur. J. Cancer,1998

2. A method for quantification of absolute amounts of nucleic acids by (RT)–PCR and a new mathematical model for data analysis;Vu;Nucleic Acids Res.,2000

3. Quantification of mRNA using real-time reverse transcription PCR (RT–PCR): trends and problems;Bustin;J. Mol. Endocrinol.,2002

4. Gene quantification using real-time quantitative PCR: an emerging technology hits the mainstream;Ginzinger;Exp. Hematol.,2002

5. Relative transcript quantification by quantitative PCR: roughly right or precisely wrong?;Skern;BMC Mol. Biol.,2005

Cited by 5 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. HSPB6 Is Depleted in Colon Cancer Patients and Its Expression Is Induced by 5-aza-2′-Deoxycytidine In Vitro;Medicina;2023-05-21

2. Cigarette Smoke Regulates the Expression of EYA4 via Alternation of DNA Methylation Status;BioMed Research International;2022-05-14

3. Integrated analysis highlights APC11 protein expression as a likely new independent predictive marker for colorectal cancer;Scientific Reports;2018-05-09

4. Selection of internal references for qRT-PCR assays of human hepatocellular carcinoma cell lines;Bioscience Reports;2017-12-22

5. Bias in the Cq value observed with hydrolysis probe based quantitative PCR can be corrected with the estimated PCR efficiency value;Methods;2010-04