Method to overcome photoreaction, a serious drawback to the use of dichlorofluorescin in evaluation of reactive oxygen species

Author:

Afzal Muhammad,Matsugo Seiichi,Sasai Masaaki,Xu Baohui,Aoyama Kohji,Takeuchi Toru

Publisher

Elsevier BV

Subject

Cell Biology,Molecular Biology,Biochemistry,Biophysics

Reference20 articles.

1. Flow cytometric studies of oxidative product formation by neutrophils: a graded response to membrane stimulation;Bass;J. Immunol.,1983

2. Flow cytometric analysis of respiratory burst activity in phagocytes with hydroethidine and 2′,7′-dichlorofluorescin;Rothe;J. Leukoc. Biol.,1990

3. In vivo visualization of oxidative changes in microvessels during neutrophil activation;Suematsu;Am. J. Physiol.,1993

4. Relationship between the intracellular reactive oxygen species and the induction of oxidative DNA damage in human neutrophil-like cells;Takeuchi;Carcinogenesis,1996

5. The antioxidant Trolox enhances the oxidation of 2′,7′-dichlorofluorescin to 2′,7′-dichlorofluorescein;Kalinich;Free Radic. Res.,1997

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