BRET-based assay to specifically monitor β2AR/GRK2 interaction and β-arrestin2 conformational change upon βAR stimulation

Author:

Parichatikanond Warisara,Kyaw Ei Thet Htar,Madreiter-Sokolowski Corina T.,Mangmool Supachoke

Funder

National Institutes of Health

Ministry of Education, Culture, Sports, Science and Technology

Publisher

Elsevier

Reference26 articles.

1. The BRET2/arrestin assay in stable recombinant cells: a platform to screen for compounds that interact with G protein-coupled receptors (GPCRs);Bertrand;Journal of Receptor and Signal Transduction Research,2002

2. Bioluminescence resonance energy transfer methods to study G protein-coupled receptor-receptor tyrosine kinase heteroreceptor complexes;Borroto-Escuela;Methods in Cell Biology,2013

3. Monitoring agonist-promoted conformational changes of beta-arrestin in living cells by intramolecular BRET;Charest;EMBO Reports,2005

4. Evolving concepts in G protein-coupled receptor endocytosis: the role in receptor desensitization and signaling;Ferguson;Pharmacological Reviews,2001

5. Role of beta-arrestin in mediating agonist-promoted G protein-coupled receptor internalization;Ferguson;Science,1996

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