An integrated protocol for targeted mutagenesis with CRISPR-Cas9 system in the pea aphid

Author:

Le Trionnaire G.,Tanguy S.,Hudaverdian S.,Gleonnec F.,Richard G.,Cayrol B.,Monsion B.,Pichon E.,Deshoux M.,Webster C.,Uzest M.,Herpin A.,Tagu D.

Funder

INRA-SPE

ANR SexAphid

Publisher

Elsevier BV

Subject

Insect Science,Molecular Biology,Biochemistry

Reference28 articles.

1. Highly efficient targeted mutagenesis of Drosophila with the CRISPR/Cas9 system;Bassett;Cell Rep.,2013

2. The Embryonic Development of Drosophila melanogaster;Campos-Ortega,2013

3. CRISPOR: intuitive guide selection for CRISPR/Cas9 genome editing experiments and screens;Concordet;Nucleic Acids Res.,2018

4. Genome sequence of the pea aphid Acyrthosiphon pisum;The International Aphid Genomics Consortium (IAGC);PLoS Biol.,2010

5. Application of the CRISPR gene‐editing technique in insect functional genome studies–a review;Cui;Entomol. Exp. Appl.,2017

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