1. Digestion mixture: 87.5% phosphate-buffered saline at pH 7.2–7.4; 10.0% trypsin solution (2.5% concentration; 1:250 activity); 0.5% serum. Optionally add 100 IU penicillin plus 100 μg streptomycin per milliliter.
2. F. J. Bové MS-222 Sandoz, p. 20. Sandoz Pharmaceuticals, Hanover, New Jersey 07936.