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The yield and quality of cellular and bacterial DNA extracts from human oral rinse samples are variably affected by the cell lysis methodology

  • Published:2016-03 Issue: Volume:122 Page:64-72
  • ISSN:0167-7012
  • Container-title:Journal of Microbiological Methods
  • language:en
  • Short-container-title:Journal of Microbiological Methods

Author:

Sohrabi Mohsen,Nair Raj G.,Samaranayake Lakshman P.,Zhang Li,Zulfiker Abu Hasanat Md.,Ahmetagic Adnan,Good David,Wei Ming Q.

Funder

Griffith University

Publisher

Elsevier BV

Subject

Microbiology (medical),Molecular Biology,Microbiology

Reference58 articles.

1. Influence of DNA extraction on oral microbial profiles obtained via 16S rRNA gene sequencing;Abusleme;J. Oral Microbiol.,2014

2. Oral microbiome profiles: 16S rRNA pyrosequencing and microarray assay comparison;Ahn;PLoS ONE,2011

3. Evaluation of methods for molecular typing and identification of members of the genus Brevibacterium and other related species;Alves;FEMS Microbiol. Lett.,2002

4. Improvement of phylum- and class-specific primers for real-time PCR quantification of bacterial taxa;Bacchetti De Gregoris;J. Microbiol. Methods,2011

5. Achromopeptidase for rapid lysis of oral anaerobic gram-positive rods;Barsotti;Oral Microbiol. Immunol.,1988

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