Improving the mutagenesis efficiency of the Kunkel method by codon optimization and annealing temperature adjustment
Author:
Funder
Sichuan Science and Technology Program
Publisher
Elsevier BV
Subject
Molecular Biology,General Medicine,Bioengineering,Biotechnology
Reference29 articles.
1. Rapid and efficient site-specific mutagenesis without phenotypic selection;Kunkel;Proc Natl Acad Sci U S A,1985
2. Efficient site-directed mutagenesis using uracil-containing DNA;Kunkel;Methods Enzymol,1991
3. Identifying specificity profiles for peptide recognition modules from phage-displayed peptide libraries;Tonikian;Nat Protoc,2007
4. Efficient construction of a large collection of phage-displayed combinatorial peptide libraries;Scholle;Comb Chem High Throughput Screen,2005
5. Generation of human single domain antibody repertoires by kunkel mutagenesis;Rouet;Methods Mol Biol,2012
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