Protamine sulfate protects exogenous DNA against nuclease degradation but is unable to improve the efficiency of bovine sperm mediated transgenesis

Author:

Alderson Jon,Wilson Ben,Laible Goetz,Pfeffer Peter,L’Huillier Phil

Publisher

Elsevier BV

Subject

Endocrinology,Animal Science and Zoology,Food Animals,General Medicine

Reference21 articles.

1. Liposome-mediated DNA uptake by sperm cells;Bachiller;Mol. Reprod. Dev.,1991

2. Foreign DNA transmission by ICSI: injection of spermatozoa bound with exogenous DNA results in embryonic GFP expression and live Rhesus monkey births;Chan;Mol. Hum. Reprod.,2000

3. Plasmid DNA molecules are protected from degradation by nucleases and shearing by aerosolisation;Crook;Gene Ther.,1996

4. DNase II in bull and ram sperm tail and mitochondria;Fisher;Arch. Androl.,1980

5. Sperm mediated transgenesis;Gandolfi;Theriogenology,2000

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