Development and evaluation of SYBR Green-I based quantitative PCR assays for herpes simplex virus type 1 whole transcriptome analysis

Author:

Garvey Cathryn E.,McGowin Chris L.,Foster Timothy P.

Funder

National Center for Research Resources

National Institute of General Medical Sciences

National Institute of Allergy and Infectious Diseases

Publisher

Elsevier BV

Subject

Virology

Reference46 articles.

1. Quantitative comparison of the HSV-1 and HSV-2 transcriptomes using DNA microarray analysis;Aguilar;Virology,2006

2. Design of a herpes simplex virus type 2 long oligonucleotide-based microarray: global analysis of HSV-2 transcript abundance during productive infection;Aguilar;Methods Mol. Biol.,2005

3. Development of a SYBR Green I based real-time RT-PCR assay for detection and quantification of bovine coronavirus;Amer;Mol. Cell. Probes,2011

4. Low copy number detection of herpes simplex virus type 1 mRNA and mouse Th1 type cytokine mRNAs by Light Cycler quantitative real-time PCR;Broberg;J. Virol. Methods,2003

5. Why the need for qPCR publication guidelines? – the case for MIQE;Bustin;Methods,2010

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