A proposed validation method for automated nucleic acid extraction and RT-qPCR analysis: An example using Bluetongue virus

Author:

Vandemeulebroucke Elise,De Clercq Kris,Van der Stede Yves,Vandenbussche Frank

Publisher

Elsevier BV

Subject

Virology

Reference31 articles.

1. A fully automated procedure for the high-throughput detection of avian influenza virus by real-time reverse transcription-polymerase chain reaction;Agüero;Avian Dis.,2007

2. Detection of the European ‘field’ strain of bluetongue virus serotype 6 by real-time RT-PCR;Batten;Vet. Microbiol,2009

3. Molecular diagnosis of viral diseases, present trends and future aspects. A view from the OIE Collaborating Centre for the application of polymerase chain reaction methods for diagnosis of viral diseases in veterinary medicine;Belak;Vaccine,2007

4. Automated extraction of viral-pathogen RNA and DNA for high-throughput quantitative real-time PCR;Beuselinck;J. Clin. Microbiol.,2005

5. Modelling the limit of detection in real-time quantitative PCR;Burns;Eur. Food Res. Technol.,2008

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