Development of a rapid, sensitive TaqMan real-time RT-PCR assay for the detection of Rose rosette virus using multiple gene targets

Author:

Babu Binoy,Jeyaprakash Ayyamperumal,Jones Debra,Schubert Timothy S.,Baker Carlye,Washburn Brian K.,Miller Steven H.,Poduch Kristina,Knox Gary W.,Ochoa-Corona Francisco M.,Paret Mathews L.

Funder

Florida Nursery Growers and Landscape Association, Dean for Research, Institute of Food and Agriculture Sciences, University of Florida

USDA-SCRI

Publisher

Elsevier BV

Subject

Virology

Reference68 articles.

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2. Multiflora rose;Amrine,2002

3. Failure to genotype herpes simplex virus by real-time PCR assay and melting curve analysis due to sequence variation within probe binding sites;Anderson;J. Clin. Microbiol.,2003

4. Comparative assessment of 5′ A/T-rich overhang sequences with optimal and sub-optimal primers to increase PCR yields and sensitivity;Arif;Mol. Biotechnol.,2013

5. Primer modification improves rapid and sensitive in vitro and field deployable assays for detection of High plains virus variants;Arif;Appl. Environ. Microbiol.,2014

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