Comparison of SARS-CoV-2 N gene real-time RT-PCR targets and commercially available mastermixes

Author:

Brown Julianne R.ORCID,O’Sullivan Denise M.ORCID,Shah DivyaORCID,Atkinson LauraORCID,Pereira Rui P.A.ORCID,Whale Alexandra S.ORCID,Busby Eloise J.,Huggett Jim F.ORCID,Harris KathrynORCID

Publisher

Elsevier BV

Subject

Virology

Reference15 articles.

1. Comparative evaluation of the Seegene Seeplex RV15 and real-time PCR for respiratory virus detection;Bibby;J. Med. Virol.,2011

2. The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments;Bustin;Clin. Chem.,2009

3. CDC Research Use Only 2019-Novel Coronavirus (2019-nCoV) Real-time RT-PCR Primers and Probes. https://www.cdc.gov/coronavirus/2019-ncov/lab/rt-pcr-panel-primer-probes.html (accessed 07/12/2020).

4. Extraction-free COVID-19 (SARS-CoV-2) diagnosis by RT-PCR to increase capacity for national testing programmes during a pandemic;Grant;bioRxiv,2020

5. ISO 15189:2012 Medical Laboratories — Requirements for Quality and Competence;International Organization for Standardization,2012

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