Proteinase K treatment in absence of RNA isolation classical procedures is a quick and cheaper alternative for SARS-CoV-2 molecular detection

Author:

Mallmann L.ORCID,Hermann B.S.ORCID,Schallenberger K.,Demoliner M.,Eisen A.K.A.,Heldt F.H.,Gularte J.S.,Hansen A.W.ORCID,de Almeida P.R.,Weber M.N.ORCID,Spilki F.R.ORCID,Fleck J.D.

Funder

Ministério da Ciência Tecnologia e Inovação

CNPq

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior

Financiadora de Estudos e Projetos

Publisher

Elsevier BV

Subject

Virology

Reference8 articles.

1. RNA Extraction Kits for COVID-19 Tests Are in Short Supply in US [WWW Document];Akst,2020

2. Interim guidelines for collecting, handling, and testing clinical specimens for COVID-19 [WWW document];CDC,2020

3. Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR;Corman;Eurosurveillance,2020

4. An alternative workflow for molecular detection of SARS-CoV-2 – escape from the NA extraction kit-shortage, Copenhagen, Denmark, March 2020;Fomsgaard;Eurosurveillance,2020

5. The species Severe acute respiratory syndrome-related coronavirus: classifying 2019-nCoV and naming it SARS-CoV-2;Gorbalenya;Nat. Microbiol.,2020

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