Diagnostic accuracy of direct reverse transcription-polymerase chain reaction using guanidine-based and guanidine-free inactivators for SARS-CoV-2 detection in saliva samples

Author:

Katsuno Takashi,Kimura Moto,Terada-Hirashima Junko,Kazuyama Yukumasa,Ikeda Masato,Moriya Ataru,Kurokawa Masami,Motohashi Ayano,Isaka Erina,Morishita Momoko,Kawajiri Kazuki,Hakkaku Kazuo,Saito Susumu,Terayama Yuriko,Sugiura Yuriko,Yamaguchi Yoh,Takumida Hiroshi,Watanabe Hiromu,Morita Chie,Tsukada Akinari,Kusaba Yusaku,Tsujimoto Yoshie,Ishida Akane,Sakamoto Keita,Hashimoto Masao,Suzuki Manabu,Takasaki Jin,Izumi Shinyu,Hojo Masayuki,Sugiyama Haruhito,Sugiura Wataru

Publisher

Elsevier BV

Reference15 articles.

1. Circadian rhythms in human salivary flow rate and composition;Dawes;J. Physiol.,1972

2. Repeated coronavirus disease 2019 molecular testing: correlation of severe acute respiratory syndrome coronavirus 2 culture with molecular assays and cycle thresholds;Gniazdowski;Clin. Infect. Dis.,2021

3. Efficacy validation of SARS-CoV-2-inactivation and viral genome stability in saliva by a guanidine hydrochloride and surfactant-based virus lysis/transport buffer;Komu;Viruses,2023

4. National Institute of Infectious Diseases, 2020. Manual for the detection of pathogen 2019-nCoV Ver.2.6. 〈https://www.niid.go.jp/niid/images/epi/corona/2019-nCoVmanual20200217-en.pdf〉 (accessed 14 January 2021).

5. National Institutes of Health. Clinical Spectrum of SARS-CoV-2 infection 〈https://www.covid19treatmentguidelines.nih.gov/overview/clinical-spectrum/〉 (accessed 15 December 2023).

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