Isolation of leukocytes from frozen buffy coat for comet assay analysis of DNA damage
Author:
Funder
Throne Holst Foundation for Nutrition Research, Norway
Publisher
Elsevier BV
Subject
Health, Toxicology and Mutagenesis,Genetics
Reference4 articles.
1. Simplified method for the collection, storage, and comet assay analysis of DNA damage in whole blood;Al-Salmani;Free Radic. Biol. Med.,2011
2. DNA damage in blood cells in relation to chemotherapy and nutritional status in colorectal cancer patients-A pilot study;Kvaerner;DNA Repair (Amst),2018
3. Leucocytes isolated from simply frozen whole blood can be used in human biomonitoring for DNA damage measurement with the comet assay;Akor-Dewu;Cell Biochem. Funct.,2014
4. Twelve-gel slide format optimised for comet assay and fluorescent in situ hybridisation;Shaposhnikov;Toxicol. Lett.,2010
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1. Measuring DNA modifications with the comet assay: a compendium of protocols;Nature Protocols;2023-01-27
2. Short- and long-term reproducibility of the COMET assay for measuring DNA damage biomarkers in frozen blood samples of the EPIC-Heidelberg cohort;Mutation Research/Genetic Toxicology and Environmental Mutagenesis;2022-02
3. CometChip analysis of human primary lymphocytes enables quantification of inter-individual differences in the kinetics of repair of oxidative DNA damage;Free Radical Biology and Medicine;2021-10
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