Efficient purification of a microbial steroid-1-dehydrogenase by electrophoretic desorption from the affinity matrix on a preparative scale
Author:
Publisher
Elsevier BV
Subject
Organic Chemistry,General Medicine,Biochemistry,Analytical Chemistry
Reference14 articles.
1. Steroidumwandelnde enzyme aus mikro-organismen—VIII. Einfluβ organischer lösungsmittel unterschiedlicher hydrophobizität auf die bindung an der affinitätsmatrix-affinitätschromatographie einer 4-en-3-oxo-steroid: (akzeptor)-1-en-oxidoreduktase aus Nocardia opaca
2. Electrophoresis: A new preparative desorption technique in affinity chromatography (and immunoadsorption)
3. Isolation of antibodies to steroid hormones by affinity chromatography on antigen-linked sepharose: An efficient electrophoretic elution procedure
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1. The role and mechanism of microbial 3-ketosteroid Δ1-dehydrogenases in steroid breakdown;The Journal of Steroid Biochemistry and Molecular Biology;2019-07
2. Transformation of steroids by actinobacteria: A review;Applied Biochemistry and Microbiology;2007-02
3. Prospects for the synthesis of hydrazine(hydrazine)-containing affinity type adsorbents (a review);Pharmaceutical Chemistry Journal;1997-10
4. Isotachophoretic desorption of anionic compounds from solid-phase adsorbents;Journal of Chromatography A;1993-12
5. Improved Purification of Steroid 1:2-Dehydrogenase from Nocardia opaca and Partial Characterization of Its Cloned Gene Sequence;Biochemical and Biophysical Research Communications;1993-01
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