Role of the gradient slope during the product internal recycling for the multicolumn countercurrent solvent gradient purification of PEGylated proteins

Author:

Kim Tae KeunORCID,Bham Abdallah AyubORCID,Fioretti Ismaele,Angelo James,Xu XuankuoORCID,Ghose Sanchayita,Morbidelli Massimo,Sponchioni MattiaORCID

Publisher

Elsevier BV

Subject

Organic Chemistry,General Medicine,Biochemistry,Analytical Chemistry

Reference41 articles.

1. Protein PEGylation for the design of biobetters: from reaction to purification processes;Santos;Braz. J. Pharm. Sci.,2018

2. Biopharma P.E.G. FDA approved PEGylated Drugs Up To 2022. Published 2019. Accessed February 14, 2022. https://www.biochempeg.com/article/58.html

3. N-terminal PEGylation of human serum albumin and investigation of its pharmacokinetics and pulmonary microvascular retention;Zhao;Biosci. Trends,2012

4. Safety of PEGylated recombinant human full-length coagulation factor VIII (BAX 855) in the overall context of PEG and PEG conjugates;Stidl;Haemophilia,2016

5. Experimental design of the multicolumn countercurrent solvent gradient purification (MCSGP) unit for the separation of pegylated proteins;Kim;Ind. Eng. Chem. Res.,2021

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