Simple and inexpensive three-step rapid amplification of cDNA 5′ ends using 5′ phosphorylated primers
Author:
Funder
Wellcome Trust
Scientific Research
KULeuven
Publisher
Elsevier BV
Subject
Cell Biology,Molecular Biology,Biochemistry,Biophysics
Reference20 articles.
1. Amplification of cDNA ends using PCR suppression effect and step-out PCR;Matz;Methods Mol. Biol.,2003
2. Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer;Frohman;Proc. Natl. Acad. Sci. USA,1988
3. A highly sensitive method for mapping the 5′ termini of mRNAs;Fromont-Racine;Nucleic Acids Res.,1993
4. Ligation-mediated amplification of RNA from murine erythroid cells reveals a novel class of beta-globin mRNA with an extended 5′-untranslated region;Volloch;Nucleic Acids Res.,1994
5. Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides;Maruyama;Gene,1994
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