High diluted and dynamised follicle stimulating hormone modulates steroid production in isolated porcine preantral follicles cultured in vitro

Author:

de Lima Laritza F1,Rubessa Marcello2,Rocha Rebeca MP1,Winters Rebecca3,Milner Derek J2,Campello Claudio C1,Figueiredo José R1,Wheeler Matthew B32

Affiliation:

1. Laboratory of Manipulation of Oocytes and Preantral Follicles, Faculty of Veterinary, State University of Ceara, Itaperi Campus, 60740-903, Fortaleza, CE, Brazil

2. Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL, United States

3. Department of Animal Sciences, University of Illinois, Urbana-Champaign, Urbana, IL, United States

Abstract

Objective: This study investigated the effect of two different follicle stimulating hormone (FSH) preparations (diluted/dynamised and diluted) on the in vitro development and steroid production (estradiol, progesterone and testosterone) of isolated porcine preantral follicle after in vitro culture. Methods: Secondary follicles were cultured in Alpha Minimum Essential Medium (α-MEM+) supplemented with grain ethanol (AL – 0.2%, v/v), diluted/dynamised FSH (rFSH 6cH – 0.05 fg/mL) or diluted-only FSH (1.5 ng/mL) for 4 days. Follicle development was evaluated on the basis of follicular growth, morphology and hormone production. Results: The percentage of follicular integrity and extrusion were not affected by the treatments after culture. For all treatments, follicular diameter increased significantly from Day 0 to Day 4. On Day 2 of culture, the estradiol production was significantly higher in AL and diluted-only FSH treatments compared with diluted/dynamised FSH. However, diluted/dynamised FSH showed a significantly higher progesterone production on Day 2. Only on Day 4, the testosterone production was higher in the AL than diluted-only FSH treatments, but similar to diluted/dynamised FSH treatment. Except for diluted/dynamised FSH treatment, progesterone production increased (P < 0.05) from Day 2 to Day 4; only for AL treatment, a significant increase of testosterone production was observed during culture. Conclusion: Compared to control the diluted/dynamised FSH addition increased progesterone production but decreased the estradiol production after in vitro culture of isolated porcine preantral follicles. Taken together the results suggest that at least for progesterone production the mechanism of action of diluted/dynamised FSH differs from its vehicle.

Publisher

Georg Thieme Verlag KG

Subject

Complementary and alternative medicine

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