Gene amplification and vector engineering to achieve rapid and high-level therapeutic protein production using the Dhfr-based CHO cell selection system
Author:
Publisher
Elsevier BV
Subject
Biotechnology,Bioengineering,Applied Microbiology and Biotechnology
Reference76 articles.
1. Rapid development of CHO cell lines for high-level production of recombinant antibodies;Allison;Bioprocess J,2003
2. Tuning genetic control through promoter engineering;Alper;Proc Natl Acad Sci,2005
3. Transgenes encompassing dual-promoter CpG islands from the human TBP and HNRPA2B1 loci are resistant to heterochromatin-mediated silencing;Antoniou;Genomics,2003
4. Cell engineering blocks stress and improves biotherapeutic production;Arden;Bioprocessing,2004
5. Rational vector design and multi-pathway modulation of HEK 293E cells yield recombinant antibody titers exceeding 1g/l by transient transfection under serum-free conditions;Backliwal;Nucleic Acids Res,2008
Cited by 82 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献
1. Development of a novel tyrosine-based selection system for generation of recombinant Chinese hamster ovary cells;Journal of Bioscience and Bioengineering;2024-01
2. Combining regulated and constitutive protein expression significantly boosts protein expression by increasing productivity without affecting CHO cell growth;Biotechnology Progress;2023-03-11
3. Production of recombinant human tumor necrosis factor receptor- IgG1 Fc domain fusion protein expressed by genetically CHO- DG44 cells;Journal of Cellular Biotechnology;2022-09-20
4. Hybrid cell line development system utilizing site-specific integration and methotrexate-mediated gene amplification in Chinese hamster ovary cells;Frontiers in Bioengineering and Biotechnology;2022-09-15
5. Engineering and validation of a dual luciferase reporter system for quantitative and systematic assessment of regulatory sequences in Chinese hamster ovary cells;Scientific Reports;2022-04-11
1.学者识别学者识别
2.学术分析学术分析
3.人才评估人才评估
"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370
www.globalauthorid.com
TOP
Copyright © 2019-2024 北京同舟云网络信息技术有限公司 京公网安备11010802033243号 京ICP备18003416号-3