Statistical experimental designs to optimize the transient transfection of HEK 293T cells and determine a transfer criterion from adherent cells to larger-scale cell suspension cultures-Reference-Cited by-同舟云学术

Statistical experimental designs to optimize the transient transfection of HEK 293T cells and determine a transfer criterion from adherent cells to larger-scale cell suspension cultures

Published:2022-02 Issue: Volume:346 Page:23-34
ISSN:0168-1656
Container-title:Journal of Biotechnology
language:en
Short-container-title:Journal of Biotechnology

Author:

Dekevic Gregor,Tasto Lars,Czermak Peter,Salzig Denise

Publisher

Elsevier BV

Subject

Applied Microbiology and Biotechnology,General Medicine,Biotechnology,Bioengineering

Reference57 articles.

1. DNA/PEI nano-particles for gene delivery of rat bone marrow stem cells;Ahn;Colloids Surf. A: Physicochem. Eng. Asp.,2008

2. High-density transfection with HEK-293 cells allows doubling of transient titers and removes need for a priori DNA complex formation with PEI;Backliwal;Biotechnol. Bioeng.,2008

3. Recombinant protein production by large-scale transient gene expression in mammalian cells: state of the art and future perspectives;Baldi;Biotechnol. Lett.,2007

4. Transfer of high copy number plasmid into mammalian cells by calcium phosphate transfection;Batard;Gene,2001

5. Disassembly of polyethylenimine-DNA particles in vitro: implications for polyethylenimine-mediated DNA delivery;Bertschinger;J. Control. Release,2006

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