Probing the role of helix α1 in the acid-tolerance and thermal stability of the Streptomyces sp. SK Glucose Isomerase by site-directed mutagenesis

Author:

Hajer Ben Hlima,Dorra Zouari Ayadi,Monia Mezghani,Samir Bejar,Nushin Aghajari

Funder

Tunisian Ministry of Higher Education

French National Scientific Research Center (CNRS)

Publisher

Elsevier BV

Subject

Applied Microbiology and Biotechnology,General Medicine,Biotechnology,Bioengineering

Reference27 articles.

1. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs;Altschul;Nucleic Acids Res.,1997

2. Belghith, S.K., Ellouz, R., Bejar, S., 2002. Polypeptides having glucose isomerase activity and acids encoding the same, US Patent 6,372,476.

3. A thermostable glucose isomerase having a relatively low optimum pH: study of activity and molecular cloning of the corresponding gene;Belghith;Biotechnol. Lett.,1998

4. Engineered Glucose Isomerase from Streptomyces sp SK is resistant to Ca2+ inhibition and Co2+ independent;Ben Hlima;J. Ind. Microbiol. Biotechnol.,2012

5. Identification of critical residues for the activity and thermostability of Streptomyces sp. SK Glucose Isomerase;Ben Hlima;Appl. Microbiol. Biotechnol.,2013

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