In situ affinity purification of his-tagged protein A from Bacillus megaterium cultivation using recyclable superparamagnetic iron oxide nanoparticles

Author:

Gädke Johannes,Kleinfeldt Lennart,Schubert Chris,Rohde Manfred,Biedendieck Rebekka,Garnweitner Georg,Krull Rainer

Funder

Lower Saxony Ministry

Publisher

Elsevier BV

Subject

Applied Microbiology and Biotechnology,General Medicine,Biotechnology,Bioengineering

Reference39 articles.

1. Metal-affinity separations: a new dimension in protein processing;Arnold;Nat. Biotechnol.,1991

2. Antimicrobial activity in the egg wax of the African cattle tick Amblyomma hebraeum (Acari: ixodidae);Arrieta;Exp. Appl. Acarol.,2006

3. Efficient purification of His-tagged proteins from insect and mammalian cells;Betz;Promega Notes,2004

4. Systems biology of recombinant protein production using Bacillus megaterium;Biedendieck;Methods Enzymol.,2011

5. Protein separations using colloidal magnetic nanoparticles;Bucak;Biotechnol. Progr.,2003

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