Tuning a MAb glycan profile in cell culture: Supplementing N-acetylglucosamine to favour G0 glycans without compromising productivity and cell growth

Author:

Blondeel Eric J.M.,Braasch Katrin,McGill Thomas,Chang David,Engel Christina,Spearman Maureen,Butler Michael,Aucoin Marc G.

Funder

NSERC Strategic Network Grants

Andrew and Margaret Stephens Scholarship for Chemical Engineering

Publisher

Elsevier BV

Subject

Applied Microbiology and Biotechnology,General Medicine,Biotechnology,Bioengineering

Reference53 articles.

1. Stable expression of chimeric heavy chain antibodies in CHO cells;Agrawal;Methods Mol. Biol.,2012

2. Metabolic control of recombinant protein N-glycan processing in NS0 and CHO cells;Baker;Biotechnol. Bioeng.,2001

3. Inhibitory effects of d-glucosamine on the growth of Walker 256 carcinosarcoma and on protein RNA, and DNA synthesis;Bekesi;Cancer Res.,1970

4. Nonselective and efficient fluorescent labeling of glycans using 2-amino benzamide and anthranilic acid;Bigge;Anal. Biochem.,1995

5. Metabolic flux analysis of hybridoma cells in different culture media using mass balances;Bonarius;Biotechnol. Bioeng.,1996

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