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Simplified gene synthesis: A one-step approach to PCR-based gene construction

  • Published:2006-07 Issue:3 Volume:124 Page:496-503
  • ISSN:0168-1656
  • Container-title:Journal of Biotechnology
  • language:en
  • Short-container-title:Journal of Biotechnology

Author:

Wu Gang,Wolf Julie B.,Ibrahim Ameer F.,Vadasz Stephanie,Gunasinghe Muditha,Freeland Stephen J.

Publisher

Elsevier BV

Subject

Applied Microbiology and Biotechnology,General Medicine,Biotechnology,Bioengineering

Reference25 articles.

1. Gene synthesis by a LCR-based approach: high-level production of leptin-L54 using synthetic gene in Escherichia coli;Au;Biochem. Biophys. Res. Commun.,1998

2. Ligation-based synthesis of oligonucleotides with block structure;Borodina;Anal. Biochem.,2003

3. Synthetic gene technology: applications to ancestral gene reconstruction and structure-function studies of receptors;Chang;Methods Enzymol.,2002

4. Combination of DNA single strand synthesis with PCR to construct mung bean trypsin inhibitor gene;Chen;Chin. J. Biotechnol.,1993

5. Construction of synthetic genes using PCR after automated DNA synthesis of their entire top and bottom strands;Ciccarelli;Nucleic Acids Res.,1991
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