Rational design of a tripartite fusion protein of heparinase I enables one-step affinity purification and real-time activity detection-Reference-Cited by-同舟云学术

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Rational design of a tripartite fusion protein of heparinase I enables one-step affinity purification and real-time activity detection

Published:2013-01 Issue:1 Volume:163 Page:30-37
ISSN:0168-1656
Container-title:Journal of Biotechnology
language:en
Short-container-title:Journal of Biotechnology

Author:

Huang Ziliang,Ye Fengchun,Zhang Chong,Chen Shuo,Chen Yin,Wu Jingjun,Togo Masahiro,Xing Xin-Hui

Funder

National Natural Science Foundation of China

Tsinghua University Initiative Scientific Research Program

Publisher

Elsevier BV

Subject

Applied Microbiology and Biotechnology,General Medicine,Biotechnology,Bioengineering

Reference48 articles.

1. Insertion of the designed helical linker led to increased expression of tf-based fusion proteins;Amet;Pharmaceutical Research,2009

2. Design of the linkers which effectively separate domains of a bifunctional fusion protein;Arai;Protein Engineering,2001

3. Conformations of variably linked chimeric proteins evaluated by synchrotron X-ray small-angle scattering;Arai;Proteins,2004

4. An investigation of oligopeptides linking domains in protein tertiary structures and possible candidates for general gene fusion;Argos;Journal of Molecular Biology,1990

5. Engineering of routes to heparin and related polysaccharides;Bhaskar;Applied Microbiology and Biotechnology,2012

Cited by 13 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Characterization of maltose‐binding protein–fused heparinases with enhanced thermostability by application of rigid and flexible linkers;Biotechnology and Applied Biochemistry;2024-07-28

2. Engineering artificial fusion naringinase for enhancing naringenin biosynthesis;Biochemical Engineering Journal;2024-05

3. α-Synuclein: A fusion chaperone significantly boosting the enzymatic performance of PET hydrolase;Chinese Journal of Chemical Engineering;2023-07

4. Engineering Artificial Fusion Naringinase for Enhancing Naringenin Biosynthesis;2023

5. Design and biocatalytic applications of genetically fused multifunctional enzymes;Biotechnology Advances;2022-11

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