Development and validation of a new triplex real-time quantitative reverse Transcriptase-PCR assay for the clinical detection of SARS-CoV-2

Author:

Gadkar Vijay J.ORCID,Goldfarb David M.ORCID,Young Virginia,Watson NicoleORCID,Al-Rawahi Ghada N.,Srigley Jocelyn A.ORCID,Tilley Peter

Publisher

Elsevier BV

Subject

Cell Biology,Molecular Biology

Reference33 articles.

1. A new coronavirus associated with human respiratory disease in China;Wu;Nature,2020

2. A novel coronavirus from patients with pneumonia in China, 2019;Zhu;N. Engl. J. Med.,2019

3. Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR;Corman;Euro Surveill.,2020

4. Improved molecular diagnosis of COVID-19 by the novel, highly sensitive and specific COVID-19-RdRp/Hel real-time reverse transcription-PCR assay validated in vitro and with clinical specimens;Chan;J. Clin. Microbiol.,2020

5. Identification of nsp1 gene as the target of SARS-CoV-2 real-time RT-PCR using nanopore whole-genome sequencing;Chan;J. Med. Virol.,2020

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