Optimizing hyaluronidase dose and plasmid DNA delivery greatly improves gene electrotransfer efficiency in rat skeletal muscle

Author:

Akerstrom ThorbjornORCID,Vedel Kenneth,Needham Josefine,Hojman Pernille,Kontou Eftychia,Hellsten Ylva,Wojtaszewski Jørgen F.P.

Funder

Danish Council for Independent Research

Novo Nordisk Research Foundation

Publisher

Elsevier BV

Subject

Biochemistry,Biophysics

Reference53 articles.

1. Gene therapy by intramuscular injection of plasmid DNA: studies on firefly luciferase gene expression in mice;Manthorpe;Hum. Gene Ther.,1993

2. Characterization of plasmid DNA transfer into mouse skeletal muscle: evaluation of uptake mechanism, expression and secretion of gene products into blood;Levy;Gene Ther.,1996

3. Long-term, high level in vivo gene expression after electric pulse-mediated gene transfer into skeletal muscle;Mir;C. R. Acad. Sci. III,1998

4. Efficiency of high- and low-voltage pulse combinations for gene electrotransfer in muscle, liver, tumor, and skin;Andre;Hum. Gene Ther.,2008

5. In vivo plasmid DNA electroporation resulted in transfection of satellite cells and lasting transgene expression in regenerated muscle fibers;Peng;Biochem. Biophys. Res. Commun.,2005

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