Determination of enzyme kinetic parameters by continuous addition of substrate to a single reaction mixture and analysis by a tangent-slope procedure
Author:
Publisher
Elsevier BV
Subject
Cell Biology,Molecular Biology,Biochemistry,Biophysics
Reference31 articles.
1. A system for automatic recording of absorbancy and its application to enzyme-catalyzed reactions
2. Accurate automatic determination of acid phosphatase substrate saturation curves
3. The analysis of progress curves for enzyme-catalysed reactions by non-linear regression
4. A simple method for calculating Km and V from a single enzyme reaction progress curve
5. A pH-rate determination of the activity-pH profile of enzymes. Application to yeast pyruvate decarboxylase demonstrating the existence of multiple ionizable groups
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