Inhibition of DNA ligase IV enhances the CRISPR/Cas9-mediated knock-in efficiency in mouse brain neurons
Author:
Funder
JSPS
KAKENHI
Takeda Science Foundation
Uehara Memorial Foundation
Ichiro Kanehara Foundation
Publisher
Elsevier BV
Subject
Cell Biology,Molecular Biology,Biochemistry,Biophysics
Reference18 articles.
1. Fluorescent protein tagging of endogenous protein in brain neurons using CRISPR/Cas9-mediated knock-in and in utero electroporation techniques;Uemura;Sci. Rep.,2016
2. High-throughput, high-resolution mapping of protein localization in mammalian brain by in vivo genome editing;Mikuni;Cell,2016
3. Developing a de novo targeted knock-in method based on in utero electroporation into the mammalian brain;Tsunekawa;Development,2016
4. DNA repair protein RAD51 enhances the CRISPR/Cas9-mediated knock-in efficiency in brain neurons;Kurihara;Biochem. Biophys. Res. Commun.,2020
5. One-step generation of mice carrying reporter and conditional alleles by CRISPR/Cas-mediated genome engineering;Yang;Cell,2013
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1. Deficiency of ligase IV leads to reduced NHEJ, accumulation of DNA damage, and can sensitize cells to cancer therapeutics;Genomics;2023-11
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4. From DNA break repair pathways to CRISPR/Cas-mediated gene knock-in methods;Life Sciences;2022-04
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