An efficient and rapid method for gene cloning from eukaryotic genomic DNA using overlap-PCR: With an example of cattle Ghrelin gene-Reference-Cited by-同舟云学术

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An efficient and rapid method for gene cloning from eukaryotic genomic DNA using overlap-PCR: With an example of cattle Ghrelin gene

Published:2010-01 Issue:3 Volume:391 Page:1490-1493
ISSN:0006-291X
Container-title:Biochemical and Biophysical Research Communications
language:en
Short-container-title:Biochemical and Biophysical Research Communications

Author:

Zhang Ai-ling,Zhang Li,Zhang Liang-zhi,Chen Hong,Lan Xian-yong,Zhang Chun-lei,Zhang Cun-fang

Funder

the National 863 Program of China

National Natural Science Foundation of China

National Key Technology R&D Program

Keystone Project of transfergene in China

Sci-Tech Innovation Program of Shaanxi Province

MaTS-Beef Cattle System, Basic and Foreland Technology Study Program of Henan Province

Publisher

Elsevier BV

Subject

Cell Biology,Molecular Biology,Biochemistry,Biophysics

Reference14 articles.

1. Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension;Horton;Gene,1989

2. Site-directed mutagenesis by overlap extension using the polymerase chain reaction;Ho;Gene,1989

3. Gene splicing by overlap extension: tailor-made genes using the polymerase chain reaction;Horton;Biotechniques,1990

4. A rapid and efficient method for site-directed mutagenesis using one-step overlap extension PCR;Urban;Nucleic Acids Res.,1997

5. Gene splicing and mutagenesis by PCR-driven overlap extension;Heckman;Nat. Protoc.,2007

Cited by 8 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Molecular characterization and expression patterns of ghrelin in the reindeer (Rangifer tarandus);Polish Journal of Veterinary Sciences;2023-07-26

2. Prokaryotic expression and solubilisation of Arabidopsis ROOT UVB SENSITIVE 1 from inclusion bodies in Escherichia coli;Botanica Serbica;2022

3. Alternative Seamless Cloning Strategies in Fusing Gene Fragments Based on Overlap-PCR;Molecular Biotechnology;2021-01-13

4. Cloning and tissue distribution of novel splice variants of the ovine ghrelin gene;BMC Veterinary Research;2014-09-06

5. An alternative method for cDNA cloning from surrogate eukaryotic cells transfected with the corresponding genomic DNA;Biotechnology Letters;2012-04-03

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