EtOH-LN cryoembedding workflow to minimize freezing artifact in frozen tissues: A pilot study in preparing tissues compatible with mass spectrometry-based spatial proteomics application

Author:

Tan Yong ChiangORCID,Mustangin Muaatamarulain,Rosli NurwahyunaORCID,Wan Ahmad Kammal Wan Syahira EllaniORCID,Md Isa Nurismah,Low Teck YewORCID,Lee Pey Yee,Chellappan Dinesh Kumar,Jarmin Razman,Zuhdi Zamri,Azman Azlanudin,Ian ChikORCID,Yusof Nursuhadah Mohamed,Lim Lay ChengORCID

Funder

Government of Malaysia Ministry of Higher Education

Publisher

Elsevier BV

Subject

General Agricultural and Biological Sciences,General Biochemistry, Genetics and Molecular Biology,General Medicine

Reference15 articles.

1. Standard techniques for formalin-fixed paraffin-embedded tissue: a Pathologist's perspective;Al-Sabaawy;Iraqi J. Vet. Sci.,2021

2. Rapid freezing of skeletal and cardiac muscles using isopentane cooled with liquid nitrogen and tragacanth gum for histological, genetic, and protein expression studies;Anwar;Muscular Dystrophy Ther. Methods Mol. Biol.,2023

3. Test of the FlashFREEZE unit in tissue samples freezing for biobanking purposes;Biskup;Cell Tissue Bank.,2023

4. Comparative evaluation of two methods for LC-MS/MS proteomic analysis of formalin fixed and paraffin embedded tissues;Davalieva;J. Proteonomics,2021

5. Factors that drive the increasing use of FFPE tissue in basic and translational cancer research;Gaffney;Biotech. Histochem.,2018

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