Affiliation:
1. Pakistan Institute of Engineering and Applied Sciences, Nilore, Islamabad 45650, Pakistan
2. National Probiotic Lab-NIBGE, Jhang Road, Faisalabad 38000 (Punjab), Pakistan
Abstract
The present study was done to explore the diversity of lactic acid bacteria (LAB) associated with the gastrointestinal tract (GIT) of honeybee species endemic to northeastern Pakistan. Healthy worker bees belonging to Apis mellifera, A. dorsata, A. cerana and A. florea were collected from hives and the surroundings of a major apiary in the region. The 16S rRNA amplicon sequencing revealed a microbial community in A. florea that was distinct from the others in having an abundance of
Lactobacillus
and Bifidobacteria. However, this was not reflected in the culturable bacteria obtained from these species. The isolates were characterized for safety parameters, and 20 LAB strains deemed safe were evaluated for resistance to human GIT stresses like acid and bile, adhesion and adhesiveness, and anti-pathogenicity. The five most robust strains,
Enterococcus saigonensis
NPL780a,
Lactobacillus rapi
NPL782a,
Lactobacillus kunkeei
NPL783a, and NPL784, and
Lactobacillus paracasei
NPL783b, were identified through normalized Pearson (n) principal components analysis (PCA). These strains were checked for inhibition of human pathogens, antibiotic resistance, osmotic tolerance, metabolic and enzymatic functions, and carbohydrate utilization, along with antioxidative and cholesterol-removing potential. The findings suggest at least three strains (NPL 783a, 784 and 782a) as candidates for further in vitro and in vivo investigations of their potential health benefits and application as novel probiotic adjuncts.
Funder
Ministry of Planning, Development & Special Initiatives, Govt. of Pakistan
Cited by
4 articles.
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