Affiliation:
1. Departamento de Bioquímica y Biología Molecular, Facultad de Veterinaria, Universidad de León, 24007 León, Spain
Abstract
Pseudomonas putida U does not degrade D-glucose through the glycolytic pathway but requires (i) its oxidation to D-gluconic acid by a peripherally located constitutive glucose dehydrogenase (insensitive to osmotic shock), (ii) accumulation of D-gluconic acid in the extracellular medium, and (iii) the induction of a specific energy-dependent transport system responsible for the uptake of D-gluconic acid. This uptake system showed maximal rates of transport at 30 ° in 50 mM potassium phosphate buffer, pH 7.0. Under these conditions the Km calculated for D-gluconic acid was 6.7 μM. Furthermore, a different transport system, specific for the uptake of glucose, was also identified. It is active and shows maximal uptake rates at 35 ° in 50 mM potassium phosphate buffer, pH 6.0, with a K
m value of 8.3 μM.
Reference63 articles.
1. Occurrence, metabolism, metabolic role, and industrial uses of bacterial polyhydroxyalkanoates.;Anderson;Microbial Rev,1990
2. Metabolism of phenol and cresols by mutants of Pseudomonas putida.;Bayly;J Bacteriol,1973
3. Strategy for the generation of 13C subspectra. Application to the analysis of the 13C spectrum of the antibiotic ristocetin.;Bendall;J Org Chem,1982
4. SFructose-6-phosphate kinase from rabbit muscle. In Methods of Enzymatic Analysis, Edited by H. U. Bergmeyer. New York:;Bergmeyer;Academic Press.,1974a
5. Gluconate kinase. In Methods of Enzymatic Analysis, Edited by H. U. Bergmeyer. New York:;Bergmeyer;Academic Press.,1974b
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