Generalized transduction in the potato blackleg pathogen Erwinia carotovora subsp. atroseptica by bacteriophage M1-Reference-Cited by-同舟云学术

Generalized transduction in the potato blackleg pathogen Erwinia carotovora subsp. atroseptica by bacteriophage M1

Published:1997-07-01 Issue:7 Volume:143 Page:2433-2438
ISSN:1350-0872
Container-title:Microbiology
language:en
Short-container-title:

Author:

Toth I. K.¹²,Mulholland V.²,Cooper V.²,Bentley S.²,Shih Y.-L.²,Perombelon M. C. M.¹,Salmond G. P. C.³

Affiliation:

1. Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, Scotland, UK

2. Department of Biological Sciences, University of Warwick, Gibbet Hill Road, Coventry CV4 7AL, UK

3. Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1QW, UK

Abstract

Summary: Using enrichment methods, a new bacteriophage (M1) was isolated, which is capable of generalized transduction in Erwinia carotovora subsp. atroseptica (Eca) strain SCRI1043. M1 is probably a virulent phage and contains double-stranded DNA of approximately 43 kb. Transduction frequencies for a number of chromosomal markers and plasmid pHCP2 were established, and conditions for transduction optimized. UV irradiation of the lysates prior to transduction enhanced the transduction frequency. M1 infected over 25% of Eca strains tested and so may be useful both for the genetic analysis of a number of Eca isolates and for the transductional transfer of selectable markers between strains.

Funder

BBSRC/CASE student with SCRI

Scottish Office Agriculture, Environment and Fisheries Department

BBSRC, UK

NERC, UK

Publisher

Microbiology Society

Subject

Microbiology

Reference23 articles.

1. Extracellular enzymes and pathogenesis of soft rot Erwinia;Barras;Annu Rev Phytopathol,1994

2. Ultrastructure of bacteriophages and bacteriocins;Bradley;Bacteriol Rev,1967