Two classes of ethidium-bromide-resistant mutants of Streptomyces lividans 66-Reference-Cited by-同舟云学术

Two classes of ethidium-bromide-resistant mutants of Streptomyces lividans 66

Published:1996-04-01 Issue:4 Volume:142 Page:1041-1047
ISSN:1350-0872
Container-title:Microbiology
language:en
Short-container-title:

Author:

Lee Li-Fong¹,Huang Yi-Jiun²,Chen Carton W.³

Affiliation:

1. Institute of Biochemistry, National Yang-Ming University, Taipei 112, Taiwan

2. Institute of Microbiology & Immunology, National Yang-Ming University, Taipei 112, Taiwan

3. Institute of Genetics, National Yang-Ming University, Taipei 112, Taiwan

Abstract

Five spontaneous mutants of Streptomyces lividans TK64 resistant to 5 or 15 μM ethidium bromide (EB) were isolated, and the corresponding mutations were mapped to two different chromosomal locations. Both types of mutations conferred unselected resistance to several basic dyes and norfloxacin. The strain with the low-level resistance exhibited wild-type levels of EB uptake and energy-dependent efflux, and the resistance mechanism is unclear. The highly resistant mutants, which additionally were resistant to phosphonium ions, had a reduced accumulation and an increased efflux of EB, reminiscent of a mammalian multidrug resistance efflux pump.

Publisher

Microbiology Society

Subject

Microbiology

Reference39 articles.

1. The theory of mutagenesis;Brenner;J Mol Biol,1961

2. Transcriptional organization and regulation of an antibiotic export complex in the producing Streptomjces culture;Caballero;Mol Gen Genet,1991

3. Plasmid curing and generation of mutations induced with ethidium bromide in streptomycetes;Crameri;J Gen Microbiol,1986

4. General nature of the genetic code for proteins;Crick;Nature,1961

Cited by 4 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Functional role of bacterial multidrug efflux pumps in microbial natural ecosystems;FEMS Microbiology Reviews;2009-03

2. A multidrug efflux system is involved in colony growth in Streptomyces lividans;Microbiology;2007-04-01

3. Repressed multidrug resistance genes in Streptomyces lividans;Archives of Microbiology;2003-09-01

4. Plasmid-Mediated Resistance to Antimicrobial Agents among Listeriae;Journal of Food Protection;1998-11-01