A neutral trehalase gene from Candida albicans: molecular cloning, characterization and disruption

Author:

Eck Raimund1,Bergmann Clemens1,Ziegelbauer Karl2,Schönfeld Wolfgang2,Künkel Waldemer1

Affiliation:

1. Hans-KnöUll-Institut für Naturstoff-Forschung e.V., Abteilung Mykologie,Beutenbergstraβe 11, D-07745 Jena,Germany

2. Bayer AG, PH-R Antiinfectiva Forschung I,D-42096 Wuppertal,Germany

Abstract

A neutral trehalase gene, NTC1, from the human pathogenic yeast Candida albicans was isolated and characterized. An ORF of 2724 bp was identified encoding a predicted protein of 907 amino acids and a molecular mass of 104 kDa. A single transcript of approxymately 3·2 kb was detected by Northern blot analysis. Comparison of the deduced amino acid sequence of the C. albicans NTC1 gene product with that of the Saccharomyces cerevisiae NTH1 gene product revealed 57% identity. The NTC1 gene was localized on chromosome 1 or R. A null mutant (Δntc1/Δntc1) was constructed by sequential gene disruption. Extracts from mutants homozygous for neutral trehalase deletion had only marginal neutral trehalase activity. Extracts from heterozygous mutants showed intermediate activities between extracts from the wild-type strain and from the homozygous mutants. The null mutant showed no significant differences in pathogenicity as compared to the wild-type strain in a mouse model of systemic candidiasis. This result indicates that the neutral trehalase of C. albicans is not a potential target for antifungal drugs.

Publisher

Microbiology Society

Subject

Microbiology

Reference39 articles.

1. Purification and characterization of neutral trehalase from the ABYS1 mutant.;App;J Biol Chem,1989

2. Evidence for regulatory trehalase activity in Candida utilis.;Arguelles;Can J Microbiol,1985

3. Trehalose and glycogen levels during the initial stages of growth of Candida albicans.;Arnold;Physiol Chem Pbys,1975

4. Effect of validamycins on glycohydrolases of Rkizoctonia solani.;Asano;J Antibiot,1987

5. Induced chromosome rearrangements and morphologic variations in Candida albicans.;Barton;J Bacterial,1993

Cited by 43 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3