Identification of species-specific and cross-reactive epitopes in human polyomavirus capsids using monoclonal antibodies-Reference-Cited by-同舟云学术

Identification of species-specific and cross-reactive epitopes in human polyomavirus capsids using monoclonal antibodies

Published:2009-03-01 Issue:3 Volume:90 Page:634-639
ISSN:0022-1317
Container-title:Journal of General Virology
language:en
Short-container-title:

Author:

Randhawa Parmjeet¹,Viscidi Raphael²,Carter Joseph J.³,Galloway Denise A.³,Culp Tim D.⁴,Huang Cathy¹,Ramaswami Bala¹,Christensen Neil D.⁴

Affiliation:

1. Department of Pathology, University of Pittsburgh, Pittsburgh, PA 15213, USA

2. Department of Pediatrics, Johns Hopkins University, Baltimore, MD, USA

3. Fred Hutchinson Cancer Research Center, University of Washington, Seattle, WA, USA

4. Department of Pathology, Jake Gittlen Cancer Research Foundation, Pennsylvania State University, Hershey, USA

Abstract

The human antibody response to polyomavirus capsid proteins is not well characterized. Recombinant BK virus (BKV), JC virus (JCV) and simian virus 40 (SV40) virus-like particles (VLP) were produced in a baculovirus system, and mouse monoclonal antibodies (mAbs) to these proteins were generated using standard methods. Nine of 12 BKV mAbs showed neutralizing activity. The non-neutralizing antibodies also bound BKV pseudocapsids in an ELISA binding assay. Most antibodies recognized conformational species-specific epitopes, but several exceptions were found: (i) BKV mAb BK-F11 cross-reacted with a linear buried epitope common to both JCV and SV40 pseudocapsids, (ii) two of six JCV antibodies (JC-6.7 and JC-7.9) and two of 13 SV40 antibodies (VP1-H2 and VP1-I2) recognized linear buried epitopes common to all three viruses and (iii) SV40 antibody VP1-E5 recognized a linear surface epitope on JCV pseudocapsids.

Publisher

Microbiology Society

Subject

Virology

Reference34 articles.

1. A combination of low-dose chlorpromazine and neutralizing antibodies inhibits the spread of JC virus (JCV) in a tissue culture model: implications for prophylactic and therapeutic treatment of progressive multifocal leukencephalopathy;Atwood;J Neurovirol,2001

2. BK virus antibody titers markedly increase with viremia;Bohl;Am J Transplant,2005

3. Lack of serologic evidence for prevalent simian virus 40 infection in humans;Carter;J Natl Cancer Inst,2003

4. Surface conformational and linear epitopes on HPV-16 and HPV-18 L1 virus-like particles as defined by monoclonal antibodies;Christensen;Virology,1996a

5. Monoclonal antibodies to HPV-6 L1 virus-like particles identify conformational and linear neutralizing epitopes on HPV-11 in addition to type-specific epitopes on HPV-6;Christensen;Virology,1996b

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