Bioactivity of Amaranthus spinosus L. leaf extracts and meals against Aeromonas hydrophila

Author:

Patalinghug Jenny Marie Remonde1ORCID,Padayao Mary Hannah Rose Abadinas1ORCID,Angeles Isagani Pablo2,Yee Jonie Calisogan1

Affiliation:

1. Department of Biology, University of San Carlos, Cebu City 6000, Philippines

2. Freshwater Fisheries Center of Cagayan Valley, Isabela State University, Echague, Philippines

Abstract

Plant-based protein is being sought after as a substitute for fish meals (powdered fish) in tilapia feeds. This is to promote sustainable aquaculture, as fish meals contribute to the dwindling marine fish catch. Amaranthus spinosus is an edible weed that shows potential to improve the growth and immunity of Nile tilapia. However, most studies only consider the survivability of fish to evaluate the benefit of using plant-based feeds and do not necessarily elucidate whether a pathogen is affected in vivo. A. spinosus leaf meals (ASLMs) were used to determine effectiveness against Aeromonas hydrophila (BIOTECH 10089) injected intraperitoneally into Nile tilapia. Formulated feeds with fish meals substituted with 50 % (ASLM50) and 75 % (ASLM75) A. spinosus leaves were fed to Nile tilapia challenged with A. hydrophila . Then spleen and kidney tissue were collected and analysed 10 days post-injection for total plate count. The fish fed with ASLM50 appeared healthier than those fed with ASLM75 and those fed with control feeds. Fish fed with ASLMs had lower A. hydrophila counts (P=0.03). Phytochemical screening and antimicrobial activity determination for crude methanolic A. spinosus leaf (ASL) and ASLMs were also conducted to enhance the in vivo results. The metabolites present in the extracts were carbohydrates, amino acids and proteins, cardiac glycosides, saponins and terpenoids. The ASL and ASLM extracts had antimicrobial activity (MIC=115 mg ml−1). Overall, the study showed that ASLMs can make tilapia more resilient against A. hydrophila infections. Fish meal substitution was best at 50 %. Higher substitution had unwanted effects (more bacterial counts), possibly due to antinutritional factors.

Publisher

Microbiology Society

Cited by 1 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3