Acquiring bifidobacteria species from formula-fed and breast-fed newborns: identifying, quantifying and creating an antibiogram

Abstract

After examining the Bifidobacterium spp. population in faeces samples from breast-fed and formula-fed infants, an antibiogram was created. The prevalence of Bifidobacterium spp. in faeces was determined using common bacterial growth media, including Man Rogos Sharpe (MRS), Brain Heart Infusion (BHI), Luria Bertani (LB) broth and Bifidobacteria agar. According to the findings, formula-fed babies had a low population of Bifidobacterium spp. in their stools while breast-fed babies had a high population. By using phylogenetic analysis of the 16S rRNA and xfp (xylose/fructose 6-phosphate phosphoketolase) genes, and RFLP mapping of Bifidobacterium isolates, it was possible to identify a new and unique Bifidobacterium species. The intensity of the reddish brown colour produced during the F6PPK (fructose 6-phosphate phosphoketolase) assay is an accurate indicator of the proportion of various bifidobacteria present. Bifidobacteria agar media produced the greatest amounts of bifidobacteria diversity and recovery. Small (SCV) and Big colony variations (BCV) were formed during growth on different media. The various antibiotic MIC values changed depending on the use of different media, growth circumstances, bile salt treatment and low pH. The findings of this study demonstrate that test conditions also impact the diversity of microbiological conditions that distinguish between resistant and susceptible bacteria.