The complete genome sequence of the avian pathogen Mycoplasma gallisepticum strain Rlow-Reference-Cited by-同舟云学术

The complete genome sequence of the avian pathogen Mycoplasma gallisepticum strain Rlow

Published:2003-09-01 Issue:9 Volume:149 Page:2307-2316
ISSN:1350-0872
Container-title:Microbiology
language:en
Short-container-title:

Author:

Papazisi Leka¹²,Gorton Timothy S.¹²,Kutish Gerald³,Markham Philip F.⁴,Browning Glenn F.⁴,Nguyen Di Kim⁵,Swartzell Steven⁶,Madan Anup⁷,Mahairas Greg⁶,Geary Steven J.¹²

Affiliation:

1. Department of Pathobiology and Veterinary Science, The University of Connecticut, Storrs, CT 06269-3089, USA

2. Center of Excellence for Vaccine Research, The University of Connecticut, Storrs, CT 06269-3089, USA

3. Plum Island Animal Disease Center, US Department of Agriculture, Greenport, NY 11944, USA

4. Department of Veterinary Science, University of Melbourne, Parkville, Victoria 3010, Australia

5. Department of Medicine, University of Washington, Seattle, WA 98195, USA

6. Regulome Corporation, Seattle, WA 98195, USA

7. The Institute for Systems Biology, Seattle, WA 98103-8904, USA

Abstract

The complete genome of Mycoplasma gallisepticum strain Rlow has been sequenced. The genome is composed of 996 422 bp with an overall G+C content of 31 mol%. It contains 742 putative coding DNA sequences (CDSs), representing a 91 % coding density. Function has been assigned to 469 of the CDSs, while 150 encode conserved hypothetical proteins and 123 remain as unique hypothetical proteins. The genome contains two copies of the rRNA genes and 33 tRNA genes. The origin of replication has been localized based on sequence analysis in the region of the dnaA gene. The vlhA family (previously termed pMGA) contains 43 genes distributed among five loci containing 8, 2, 9, 12 and 12 genes. This family of genes constitutes 10·4 % (103 kb) of the total genome. Two CDSs were identified immediately downstream of gapA and crmA encoding proteins that share homology to cytadhesins GapA and CrmA. Based on motif analysis it is predicted that 80 genes encode lipoproteins and 149 proteins contain multiple transmembrane domains. The authors have identified 75 proteins putatively involved in transport of biomolecules, 12 transposases, and a number of potential virulence factors. The completion of this sequence has spawned multiple projects directed at defining the biological basis of M. gallisepticum.

Publisher

Microbiology Society

Subject

Microbiology

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